Composite

Part:BBa_K2924034:Design

Designed by: Andreas Nakielski   Group: iGEM19_Duesseldorf   (2019-10-16)


Pcpc560 + α-s1-casein + T1/T7 Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1204
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part strongly expresses a milk protein in Synechocystis. The strength of the promoter in other organisms was not tested.

Source

The plasmid and the promoter were provided by Anna Behle1, the a-s1-casein gene was synthesized by IDT.

References

1: Behle, A., Saake P., Axmann, I. M. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948.